Efek Gastroprotektif Bawang Prei (Allium fistulosum) terhadap Gastropati pada Lambung Tikus Wistar (Rattus norvegicus) yang Diinduksi Indometasin

Ainun Nizar Masbuchin, Nurdiana Nurdiana, Bagus Putu Suryana

Abstract


OAINS adalah obat yang digunakan secara luas sebagai antipiretik, antiinflamasi, dan analgesik. Namun demikian, OAINS dapat menyebabkan gastropati dan bahkan tukak lambung jika digunakan dalam jangka waktu yang lama. Quercetin, kaempferol, dan luteolin telah diketahui memiliki efek gastroprotektif. Zat tersebut  terkandung di dalam bawang prei  (Allium fistulosum). Penelitian ini  bertujuan untuk mengetahui efek gastroprotektif bawang prei. Tikus dikelompokkan menjadi lima kelompok yaitu kelompok kontrol negatif (K1), kontrol positif (K2), dan kelompok perlakuan (K3, K4, K5). K1 dan K2 diberi normal salin, sedangkan  K3, K4, dan K5 diberi ekstrak bawang prei dengan dosis 6 mg/kgBB, 12 mg/kgBB, dan 24 mg/kgBB. Tiga puluh menit kemudian K2, K3, K4, dan K5 diberi indometasin 30 mg/kgBB. Tikus kemudian dianestesi menggunakan eter dan dibedah. Gastropati dinilai berdasarkan skor makroskopik dan skor mikroskopik. Hasil uji one way ANOVA menunjukkan bahwa terdapat perbedaan bermakna pada semua kelompok (p < 0,05).  Skor makroskopik menunjukkan bahwa K5 memiliki perbedaan yang bermakna dengan K2, K3, dan K4, namun tidak ada perbedaan yang signifikan antara K2, K3, dan K4. Skor mikroskopik menunjukkan bahwa K2 memiliki perbedaan yang bermakna dengan K3, K4, dan K5. Pada uji korelasi Pearson didapatkan nilai p = 0,000 (R = -0,917 untuk skor makroskopik, R = -0,862 untuk skor mikroskopik). Dapat disimpulkan bahwa bawang prei dapat menurunkan skor makroskopik dan mikroskopik gastropati pada tikus yang diinduksi indometasin.

 

Kata kunci: Bawang prei (Allium fistulosum), Gastroprotektif, Kaempferol, Luteolin, OAINS, Quercetin.

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References


Schlansky B, Hwang JH. Prevention Of Non-Steroidal Antiinflammatory Drug-Induced Gastropathy. J Gastroenterology. 2009; 44:44-52.

Hirlan. Gastritis. Dalam: Buku Ajar Ilmu Penyakit Dalam. Jakarta: Perhimpunan Dokter Spesialis Penyakit Dalam Indonesia. 2009.

Wolfe MMl, Lichtenstein DR, Singh G. Gastrointestinal Toxicity of Nonsteroidal Antiinflamatory Drugs (Review Article). The New England Journal of Medicine. 1999.

Katzung GB. Basic and Clinical Pharmacology. 11th Edition. Mc-Graw-Hill. 2007.

Singh G, Ramey DR, Triadafilopoulus G, Brown BW, Balise RR. GI SCORE: a Simple Self-Assessment Instrument to Quantify the Risk of Serious OAINS-Related GI Complications in RA and OA. (Abstract). Arthritis Rheum. 1998; 41:Suppl:S75.

Taha AS, Hudson N, Hawkey CJ et al. Famotidine for the Prevention of Gastric and Duodenal Ulcers Caused by Nonsteroidal Anti-Inflammatory Drugs. N Engl J Med. 1996; 334:1435-9.

Westbrook JI, Talley NJ. Diagnostic Investigation Rates and Use of Prescription Andnon-Prescription Medications Amongst Dyspeptics: A Population-Based Study of 2300 Australians. Aliment Pharmacol Ther. 2003; 17: 1171–1178.

Al-Mofleh I, Alhaider A, Mossa J, Al-Sohaibani M, Qureshi S, Rafatullah S. Antisecretagogue, Antiulcer and Cytoprotective Effects of 'Peppermint' Mentha piperita L. in Laboratory Animals. J Med Sci. 2006; 6(6):930-936.

Miean KH, Mohamed S. Flavonoid (Myrecetin, Quercetin, Kaempferol, Luteolin, and Apigenin) Content of Edible Tropical Plants. J Agric Food Chem. 2001; 49(6):3106-12

Narayana KR, Reddy MS, Chaluvadi MR, Krishna DR. Bioflavonoids Classification, Pharmacological, Biochemical Effects and Therapeutic Potential. Indian Journal of Pharmacology. 2001; 33:2-16.

Freitas CS et al. Inhibition of H+/K+ ATPase in the Gastroprotective Effect of Baccharis illinita DC. JPP. 2008; 60:1105–1110.

Goel RK, Sairam K. Antiulcer Drugs from Indigenous Sources with Emphasis on Musa sapientum, Thamrabasma, Asparagus racemosus and Zingiber officinale. Indian Journal of Pharmacology. 2002; 34:100-110.

Goel RK, Maiti RN, Tavares IA. Role of Endogenous Eicosanoids in the Antiulcer Effect of Kaempferol. 1996; 67(6):548-552.

Grupta VK, Kumria R, Garg M, Gupta M. Recent Updates on Free Radical Scavenging Flavonoids: An Overview. Asian Journal of Plants Sciences. 2010; 9(3):108-117.

Ruiz C et al. Helicobacter pylori EstV: Identification, Cloning, and Characterization of the First Lipase Isolated from an Epsilon-Proteobacterium. Appl Environ Microbiol. 2007; 73(8):2423–2431.

Alternative Medicine Review. Monograph Quercetin. Alternative Medicine Review. 1998; 3(2):140-3.

Coskun O, Kanter M, Cetin K, Kaybolmaz B, Yazgan O. Protective Effects of Quercetin, a Flavonoid Antioxidant, in Absolute Ethanol-Induced Acute Gastric Ulcer. Eur J Gen Med. 2004; 1(3):37-42.

Suzuki Y, Ishihara M, Segami T, Ito M. Anti-Ulcer Effects of Antioxidants, Quercetin, Alpha-Tocopherol, Nifedipine and Tetracycline (Abstract). Jpn J Pharmacol. 1998; 78:435-41.

Mota KSL, Dias GEN, Pinto MEF, Luiz-Ferrira A, Souza-Brito ARM, Hiruma-Lima CA et al. Flavonoids with Gastroprotective Activity. Molecules. 2009; 14:979-1012.

Wibawanto I. Uji Efektivtas Ekstrak Daun Mimba (Azadirachta indica A Juss) dalam Mengurangi Jumlah Lesi Perdarahan pada Lambung Tikus Wistar (Rattus norvegicus strain Wistar) yang Diinduksi Indometasin. Tugas Akhir. Malang: Universitas Brawijaya. 2011.

Nafeeza MI, Fauzee AM, Kamsiah J, Gapor BC. Comparative Effects of A Tocotrienol-Rich Fraction and Tocopherol in Aspirin-Induced Gastric Lesions in Rats. Asia Pacific J Clin Nutr. 2002; 11(4):309–313.

Sibilia V, Rindi F, Pagani D, Rapetti V, Locatelli A, Torsello N et al. Ghrellin Protects Against Ethanol-Induced Gastritis in Rats: Studies of Mechanism of Action. Endocrinology. 2003; 144(1):353-359.

Hiruma-Lima C et al. Antiulcerogenic Activity of Alchornea castaneafolia: Effects of Somastatin, Gastrin, Prostaglandin. Journal of Ethnopharmacology. 2006; 104:215-224.

Dengiz GO, Odabasoglu F, Halici Z, Suleyman H, Cadirci E, Bayir Y. Gastroprotective and Antioxidant Effects of Amiodarone on Indomethacin-Induced Gastric Ulcers in Rats. Arch Pharm Res. 2007; 30(11):1426-1434.

Schoen RT, Vender RJ. Mechanisms of Nonsteroidal Anti-Inflammatory Drug-Induced Gastric Damage. Am J Med. 1989; 86:449-58.

Khandare AR, Gulecha VS, Mahajan MS, Mundada AS, Gangrude MM. Evaluation of Antiulcer Activity Polyherbal Formulation. International Journal of Pharma Research and Development. 2009.

Lastra A, Martín MJ, Motilva V. Antiulcer and Gastroprotective Effects of Quercetin: A Gross and Histologic Study. (Abstract). International Journal of Experimental Research and Clinical Pharmacology. 1994; 48(1).

Nikolovska-Coleska Z et al. Identification of Phenolic Constituen Isolated from Macedonian Propolis. Bulletin of Chemist and Technologists of Macedonia. 1995; 14(1):13-17.

Kalogeromitros D et al. A Quercetin Containing Supplement Reduces Niacin-Induced Flush In Human. International Journal of Immunopathology and Pharmacology. 2008; 21(3): 509-514.

Coelho RG et al. Phytochemical Study and Antiulcerogenic Activity of Syngonanthus bisulcatus (Eriocaulaceae). Brazilian Journal of Pharmaceutical Sciences. 2006; 42(3).

Odontuya G, Hoult JRS, Houghton PJ. Structure-Activity Relationship for Antiinflammatory Effect of Luteolin and Its Derived Glycosides. Phytother Res. 2005; 19:782–786.


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